To some degree yes. Different tubes will change the tone, but do not expect very dramatic change, still something you will hear for sure when doing A/B-tests.
Im loosing my faith in new production tubes as their quality seems to be crap and it definitely cannot be quaranteed that the two of a same brand's tubes will sound identical. However, also sometimes they do sound more-or-less identical. Life span will depend a lot. Some pres last very long time (years) and some might get microphonic or have some other malfunction in hours. So it really depends and if you are about to have live shows or recording sessions it is a good idea to have a spare set.